At the IGC, our goal is to provide the research community with access to cutting-edge sequencing platforms. To this end, we offer long-read sequencing via: Oxford Nanopore sequencing on the PromethION platform and PacBio sequencing on the Revio system.
Sequencing on these platforms combines long read lengths with exceptional per-base accuracy, enabling comprehensive characterization of complex genomes, structural variants, repeat expansions, isoform diversity, epigenetic modifications, and de novo genome assemblies.
Oxford Nanopore Sequencing on the PromethION Platform
The Oxford Nanopore’s unique direct molecule sequencing platform is based on protein nanopores set in a polymer membrane. Current is passed through the nanopore, and as DNA/RNA is passed through the pore, a disruption in current is detected. PromethION runs can generate ~100Gb of data. The new kit 14 chemistry produces a median raw read accuracy of Q20+ (99%+), a dramatic improvement over previous chemistries
Applications include:
- Rapid sequence identification
- Utilizing long reads for improved genome assembly
- Analysis of full length RNA transcripts from cDNA (PCR and PCR-free)
- Direct sequencing of RNA molecules
- Metagenomic analysis
- Structural variant detection
- Copy Number detection in complex regions
PacBio Sequencing on the Revio System
PacBio sequencing utilizes Single Molecule, Real-Time (SMRT) technology to generate highly accurate HiFi long reads by repeatedly sequencing circularized DNA molecules. The Revio system delivers high-throughput long-read sequencing with HiFi read accuracies exceeding Q30 (99.9%), making it particularly well suited for applications requiring both read length and precision. PacBio long-read sequencing is increasingly used across human genomics, cancer research, transcriptomics, metagenomics, and rare disease studies where conventional short-read approaches may have limitations.
Applications include:
- Targeted sequencing to study genes at scale
- Utilizing small amounts of DNA (500 ng per sample)
- Variant calling – SVs
- Variant calling – indels
Pricing
Library prep:
Prices vary by type. For more information, click here.
Sequencing:
Nanopore: varies by application, please enquire
PacBio WGS: $245 (includes Femto and library prep)
Data yield: varies by application
30X human whole genome: $2000/sample
Additional Questions or Ready to Get Started? Contact David Mohr, Director High Throughput Sequencing