We currently feature the MiSeqi100 for smaller sequencing projects.
We do our best to sequence samples in a timely manner, but our first priority is producing high quality data.
Pricing
MiSeq i100 kits come in multiple configurations. They can be run in single or paired end mode, and include extra cycles for indexing.
| Run Type | Cost | Yield (paired end reads) |
|---|---|---|
| 5M 300 | $700 | 10 million |
| 5M 600 | $900 | 10 million |
| 25M 100 | $800 | 50 million |
| 25M 300 | $1,200 | 50 million |
| 25M 600 | $1,400 | 50 million |
| 50M 100 | $1,000 | 100 million |
| 50M 300 | $1,400 | 100 million |
| 50M 600 | $1,700 | 100 million |
| 100M 100 | $1,300 | 200 million |
| 100M 300 | $1,700 | 200 million |
Data Yield is dependent upon several factors:
Read Length
The longer the read, the more data.
Read Type
Paired end reads yield twice the data as single read.
Optimal Cluster Density
It is imperative to accurately quantitate your library to ensure high data yield. We do our best to QC libraries before sequencing, but we cannot pool samples for you.
High Quality Library
Libraries that contain a high level of adapter dimers will yield significantly less data, as the fragments will hybridize to the flowcell. Similarly, over amplified libraries can negatively impact yield.
Uniform Base Composition
Libraries that have uneven base composition tend to pose problems with the MiSeq analysis software. These issues can be mitigated using several strategies, but the net effect will be lower data yield per lane than a balanced library.
Please see Illumina’s MiSeq Specification page for current data yields.
Data Delivery
Data will be returned in Sanger FASTQ format via our high speed aspera server.